Abstract

The present study was conducted to evaluate the anti-inflammatory activity of Crocus sativus extract in various experimental models of inflammation in rats. The extract was administered orally at doses of 400 mg/kg and 600 mg/kg, and its effects were compared with a standard anti-inflammatory drug, diclofenac (10 mg/kg). Inflammatory responses were induced using models such as carrageenan, histamine, dextran, serotonin, formaldehyde, and cotton pellet granuloma. 
Biochemical parameters including total protein, albumin, acid phosphatase (ACP), and alkaline phosphatase (ALP) were also assessed to understand the systemic effects of the extract. The results showed that the 600 mg/kg dose significantly increased total protein levels without altering albumin concentrations, while ACP levels remained stable, suggesting no harmful cellular stress. A significant reduction in ALP levels was observed in the 400 mg/kg group, indicating potential liver-protective effects.
Overall, the Crocus sativus extract demonstrated considerable anti-inflammatory activity with minimal adverse effects, in contrast to diclofenac, which showed elevated liver enzyme levels. These findings suggest that the extract, particularly at 600 mg/kg, may serve as a safer and effective alternative for managing inflammation.